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human nrg ad03  (Assay Designs Inc)

 
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    Assay Designs Inc human nrg ad03
    Human Nrg Ad03, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+nrg+ad03/human+nrg+ad03+antibody/10__1074_slash_jbc__m109__032714-89-10-14
    Average 90 stars, based on 1 article reviews
    human nrg ad03 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain
    Article Snippet: Sectionswere incubatedwith antibodies to chicken NRG 183N (9) (1:100) or humanNRGAD03 (10) (21) (1:300, Assay Designs Inc., Ann Arbor, MI) and Schwann cells 1E8 (1:10, Developmental Studies Hybridoma Bank, UIowa) orHis6 tag (1:100, Abcam, Cambridge,MA) in blocking solution (10% normal goat serum, 0.5% Triton X-100 in PBS) for overnight at 4 °C, followed by incubation with goat antimouse or anti-rabbit Alexa fluor 546 (1:250, Invitrogen).

    Incubation:

    Article Title: Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain
    Article Snippet: .. Sections were incubated with antibodies to chicken NRG 183N ( 9 ) (1:100) or human NRG AD03 ( 10 ) ( 21 ) (1:300, Assay Designs Inc., Ann Arbor, MI) and Schwann cells 1E8 (1:10, Developmental Studies Hybridoma Bank, UIowa) or His 6 tag (1:100, Abcam, Cambridge, MA) in blocking solution (10% normal goat serum, 0.5% Triton X-100 in PBS) for overnight at 4 °C, followed by incubation with goat anti-mouse or anti-rabbit Alexa fluor 546 (1:250, Invitrogen). .. For AD03 or His tag immunostaining, biotin-conjugated goat anti-rabbit (1:500,PerkinElmer Life Sciences) or horseradish peroxidase-conjugated goat anti-mouse (1:100, Invitrogen) was used as secondary antibody, and signal was detected using a tyramide signal amplification kit (Invitrogen) following the manufacturer's instructions.

    Blocking Assay:

    Article Title: Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain
    Article Snippet: .. Sections were incubated with antibodies to chicken NRG 183N ( 9 ) (1:100) or human NRG AD03 ( 10 ) ( 21 ) (1:300, Assay Designs Inc., Ann Arbor, MI) and Schwann cells 1E8 (1:10, Developmental Studies Hybridoma Bank, UIowa) or His 6 tag (1:100, Abcam, Cambridge, MA) in blocking solution (10% normal goat serum, 0.5% Triton X-100 in PBS) for overnight at 4 °C, followed by incubation with goat anti-mouse or anti-rabbit Alexa fluor 546 (1:250, Invitrogen). .. For AD03 or His tag immunostaining, biotin-conjugated goat anti-rabbit (1:500,PerkinElmer Life Sciences) or horseradish peroxidase-conjugated goat anti-mouse (1:100, Invitrogen) was used as secondary antibody, and signal was detected using a tyramide signal amplification kit (Invitrogen) following the manufacturer's instructions.



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